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non targeting negative control sequence ntc  (New England Biolabs)


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    New England Biolabs non targeting negative control sequence ntc
    HEK293 cells were transfected with expression plasmids encoding mCherry with a 3′ partial gelsolin sequence and pSM30 containing inserts for miR-133a, miR-133a(v), a random non-targeting sequence <t>(NTC)</t> <t>or</t> <t>siRNA</t> against mCherry (siR-mCh). Cells were imaged and analysed for both green and red fluorescence intensity. A decrease in red/green ratio vs NTC indicates downregulation of target gene expression. Data were log transformed and compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. All pairwise comparisons were significant (p<.001). Comparison of miR-133a vs miR-133a(v) is indicated (***). Number of cells (n): miR-133a 4640; miR-133a(v) 2843; NTC 4088; siR-mCh 3884. Representative of 3 separate experiments in which miR-133a(v) was significantly more effective than miR-133a.
    Non Targeting Negative Control Sequence Ntc, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33702 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+targeting+negative+control+sequence+ntc/T4+DNA+Ligase/pmc03683050-145-31-43
    Average 99 stars, based on 33702 article reviews
    non targeting negative control sequence ntc - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Distinctive Profile of IsomiR Expression and Novel MicroRNAs in Rat Heart Left Ventricle"

    Article Title: Distinctive Profile of IsomiR Expression and Novel MicroRNAs in Rat Heart Left Ventricle

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0065809

    HEK293 cells were transfected with expression plasmids encoding mCherry with a 3′ partial gelsolin sequence and pSM30 containing inserts for miR-133a, miR-133a(v), a random non-targeting sequence (NTC) or siRNA against mCherry (siR-mCh). Cells were imaged and analysed for both green and red fluorescence intensity. A decrease in red/green ratio vs NTC indicates downregulation of target gene expression. Data were log transformed and compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. All pairwise comparisons were significant (p<.001). Comparison of miR-133a vs miR-133a(v) is indicated (***). Number of cells (n): miR-133a 4640; miR-133a(v) 2843; NTC 4088; siR-mCh 3884. Representative of 3 separate experiments in which miR-133a(v) was significantly more effective than miR-133a.
    Figure Legend Snippet: HEK293 cells were transfected with expression plasmids encoding mCherry with a 3′ partial gelsolin sequence and pSM30 containing inserts for miR-133a, miR-133a(v), a random non-targeting sequence (NTC) or siRNA against mCherry (siR-mCh). Cells were imaged and analysed for both green and red fluorescence intensity. A decrease in red/green ratio vs NTC indicates downregulation of target gene expression. Data were log transformed and compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. All pairwise comparisons were significant (p<.001). Comparison of miR-133a vs miR-133a(v) is indicated (***). Number of cells (n): miR-133a 4640; miR-133a(v) 2843; NTC 4088; siR-mCh 3884. Representative of 3 separate experiments in which miR-133a(v) was significantly more effective than miR-133a.

    Techniques Used: Transfection, Expressing, Sequencing, Fluorescence, Transformation Assay

    Related Articles

    Sequencing:

    Article Title: Distinctive Profile of IsomiR Expression and Novel MicroRNAs in Rat Heart Left Ventricle
    Article Snippet: .. Complementary pairs of oligonucleotides (25 μM) encoding artificial miRNA sequences (miR-133a canonical isomiR and most common isomiR (miR-133a(v))), a siRNA sequence designed to down regulate mCherry expression (siR-mCh), or a random non-targeting negative control sequence (NTC) were annealed and ligated (T4 DNA ligase; New England Biolabs, Hitchin, UK) into pSM30 restricted with Bsm BI (New England Biolabs, Hitchin, UK). ..

    Expressing:

    Article Title: Distinctive Profile of IsomiR Expression and Novel MicroRNAs in Rat Heart Left Ventricle
    Article Snippet: .. Complementary pairs of oligonucleotides (25 μM) encoding artificial miRNA sequences (miR-133a canonical isomiR and most common isomiR (miR-133a(v))), a siRNA sequence designed to down regulate mCherry expression (siR-mCh), or a random non-targeting negative control sequence (NTC) were annealed and ligated (T4 DNA ligase; New England Biolabs, Hitchin, UK) into pSM30 restricted with Bsm BI (New England Biolabs, Hitchin, UK). ..

    Negative Control:

    Article Title: Distinctive Profile of IsomiR Expression and Novel MicroRNAs in Rat Heart Left Ventricle
    Article Snippet: .. Complementary pairs of oligonucleotides (25 μM) encoding artificial miRNA sequences (miR-133a canonical isomiR and most common isomiR (miR-133a(v))), a siRNA sequence designed to down regulate mCherry expression (siR-mCh), or a random non-targeting negative control sequence (NTC) were annealed and ligated (T4 DNA ligase; New England Biolabs, Hitchin, UK) into pSM30 restricted with Bsm BI (New England Biolabs, Hitchin, UK). ..



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    non targeting negative control sequence ntc  (New England Biolabs)
    99
    New England Biolabs non targeting negative control sequence ntc
    HEK293 cells were transfected with expression plasmids encoding mCherry with a 3′ partial gelsolin sequence and pSM30 containing inserts for miR-133a, miR-133a(v), a random non-targeting sequence <t>(NTC)</t> <t>or</t> <t>siRNA</t> against mCherry (siR-mCh). Cells were imaged and analysed for both green and red fluorescence intensity. A decrease in red/green ratio vs NTC indicates downregulation of target gene expression. Data were log transformed and compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. All pairwise comparisons were significant (p<.001). Comparison of miR-133a vs miR-133a(v) is indicated (***). Number of cells (n): miR-133a 4640; miR-133a(v) 2843; NTC 4088; siR-mCh 3884. Representative of 3 separate experiments in which miR-133a(v) was significantly more effective than miR-133a.
    Non Targeting Negative Control Sequence Ntc, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+targeting+negative+control+sequence+ntc/T4+DNA+Ligase/pmc03683050-145-31-43
    Average 99 stars, based on 1 article reviews
    non targeting negative control sequence ntc - by Bioz Stars, 2026-10
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    HEK293 cells were transfected with expression plasmids encoding mCherry with a 3′ partial gelsolin sequence and pSM30 containing inserts for miR-133a, miR-133a(v), a random non-targeting sequence (NTC) or siRNA against mCherry (siR-mCh). Cells were imaged and analysed for both green and red fluorescence intensity. A decrease in red/green ratio vs NTC indicates downregulation of target gene expression. Data were log transformed and compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. All pairwise comparisons were significant (p<.001). Comparison of miR-133a vs miR-133a(v) is indicated (***). Number of cells (n): miR-133a 4640; miR-133a(v) 2843; NTC 4088; siR-mCh 3884. Representative of 3 separate experiments in which miR-133a(v) was significantly more effective than miR-133a.

    Journal: PLoS ONE

    Article Title: Distinctive Profile of IsomiR Expression and Novel MicroRNAs in Rat Heart Left Ventricle

    doi: 10.1371/journal.pone.0065809

    Figure Lengend Snippet: HEK293 cells were transfected with expression plasmids encoding mCherry with a 3′ partial gelsolin sequence and pSM30 containing inserts for miR-133a, miR-133a(v), a random non-targeting sequence (NTC) or siRNA against mCherry (siR-mCh). Cells were imaged and analysed for both green and red fluorescence intensity. A decrease in red/green ratio vs NTC indicates downregulation of target gene expression. Data were log transformed and compared by one-way ANOVA with Bonferroni's Multiple Comparison Test. All pairwise comparisons were significant (p<.001). Comparison of miR-133a vs miR-133a(v) is indicated (***). Number of cells (n): miR-133a 4640; miR-133a(v) 2843; NTC 4088; siR-mCh 3884. Representative of 3 separate experiments in which miR-133a(v) was significantly more effective than miR-133a.

    Article Snippet: Complementary pairs of oligonucleotides (25 µM) encoding artificial miRNA sequences (miR-133a canonical isomiR and most common isomiR (miR-133a(v))), a siRNA sequence designed to down regulate mCherry expression (siR-mCh), or a random non-targeting negative control sequence (NTC) were annealed and ligated (T4 DNA ligase; New England Biolabs, Hitchin, UK) into pSM30 restricted with Bsm BI (New England Biolabs, Hitchin, UK).

    Techniques: Transfection, Expressing, Sequencing, Fluorescence, Transformation Assay